miRNAs in Neurological Manifestation in Patients Co-Infected with SARS-CoV-2 and Herpesvírus 6 (HHV-6).
Vanessa Cristine de Souza CarneiroOtacílio da Cruz MoreiraWagner Luis da Costa Nunes Pimentel CoelhoBeatriz Chan RioDmitry José De Santana SarmentoAndreza Lemos SalvioSoniza Vieira Alves-LeonVanessa Salete de PaulaLuciane Almeida Amado LeonPublished in: International journal of molecular sciences (2023)
Human herpesviruses (HHVs) can establish latency and be reactivated, also are neurotropic viruses that can trigger neurological disorders. HHV-6 is a herpesvirus that is associated with neurological disorders. Studies have reported the detection of HHV-6 in patients with COVID-19 and neurological manifestations. However, specific diagnoses of the neurological disorders caused by these viruses tend to be invasive or difficult to interpret. This study aimed to establish a relationship between miRNA and neurological manifestations in patients co-infected with COVID-19 and HHV-6 and evaluate miRNAs as potential biomarkers. Serum samples from COVID-19 patients in the three cohorts were analyzed. miRNA analysis by real-time polymerase chain reaction (qPCR) revealed miRNAs associated with neuroinflammation were highly expressed in patients with neurological disorders and HHV-6 detection. When compared with the group of patients without detection of HHVs DNA and without neurological alterations, the group with detection of HHV-6 DNA and neurological alteration, displayed significant differences in the expression of mir-21, mir-146a, miR-155 and miR-let-7b ( p < 0.01). Our results reinforce the involvement of miRNAs in neurological disorders and provide insights into their use as biomarkers for neurological disorders triggered by HHV-6. Furthermore, understanding the expression of miRNAs may contribute to therapeutic strategies.
Keyphrases
- sars cov
- end stage renal disease
- cell proliferation
- long non coding rna
- ejection fraction
- chronic kidney disease
- newly diagnosed
- cerebral ischemia
- poor prognosis
- peritoneal dialysis
- long noncoding rna
- endothelial cells
- traumatic brain injury
- patient reported outcomes
- label free
- binding protein
- subarachnoid hemorrhage
- real time pcr
- single molecule
- lipopolysaccharide induced