GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system.
Jana NeuholdKatharina RadakovicsAnita LehnerFlorian WeissmannMaria Queralt GarciaMari Carmen RomeroNicholas S BerrowPeggy Stolt-BergnerPublished in: BMC biotechnology (2020)
This robust, single-step cloning system provides an easy-to-use method for generation of multi-gene expression constructs for both transposition and homologous recombination-based baculovirus systems, making this technology available across all laboratories using baculovirus expression systems. This highly efficient and simple method allows for rapid incorporation of multi-gene expression cloning into the standardized service portfolio of protein production facilities and can also easily be adopted by any laboratory for routine generation of multi-gene baculovirus constructs.