From Nanovesicles to Nanobubbles Based on Repeated Compression Method.

Nanobubbles have been increasingly applied in biomedicine, which is attributed to their ability to work as ultrasound imaging contrast agents and powerful gene/drug carriers. Different production techniques or approaches have been developed to generate uniform and stable shelled nanobubbles. However, these shelled nanobubbles are usually prepared based on disordered shell materials, such as free phospholipids and polymers. In recent years, the continuous repeated compression method for a gas-liquid mixture has been developed to produce free and lipid-shelled nanobubbles. In this study, to explore the response of well-organized nanostructures to this method, the repeated compression method was used to treat preprepared liposomes and polymeric nanovesicles. Size distribution, morphologies, and ultrasound image contrast enhancement of these nanovesicles were determined before and after repeated compression. Results demonstrate that the presence of a phospholipid bilayer is vital to form liposome-based nanobubbles. And the low elastic modulus of the polymeric membrane is key to encapsulate gases into polymeric nanovesicles. Overall, it demonstrated the advantages of well-organized nanostructures to produce nanobubble structures, giving new insights into the preparation and understanding of nanobubbles.