Increment of Lysosomal Biogenesis by Combined Extracts of Gum Arabic, Parsley, and Corn Silk: A Reparative Mechanism in Mice Renal Cells.
Aya HelmyMohamed El-ShazlyNesreen Nabil OmarMohamed RabehUsama Ramadan AbdelmohsenReham TashMohammad Alaraby SalemAhmed SamirAli ElshamyAbd El-Nasser B SingabPublished in: Evidence-based complementary and alternative medicine : eCAM (2020)
Gum Arabic (GA), parsley, and corn silk have been traditionally used for renal failure patients worldwide. This study aimed at probing the mechanism of the combined extracts, namely, GA (3 g/kg/day), parsley (1 g/kg/day), and corn silk (200 mg/kg/day), as nephroprotective agents in mice after amikacin (1.2 g/kg) single dose through exploration of their action on G-protein coupled receptors (GPR) 41 and 43 and the ensuing lysosomal biogenesis. Western blotting was employed for renal levels of bcl-2-associated X protein (BAX) and cytosolic cathepsin D; cell death markers, nuclear transcription factor EB (TFEB), and lysosomal associated membrane protein-1 (LAMP-1); and lysosomal biogenesis indicators. Liquid chromatography-mass spectrometry (LC-MS) and docking were also employed. After amikacin treatment, BAX and cathepsin D levels were upregulated while LAMP-1 and nuclear TFEB levels were inhibited. The combined extracts inhibited BAX and cytosolic cathepsin D but upregulated LAMP-1 and nuclear TFEB levels. Docking confirmed GPR modulatory signaling. The combined extracts showed GPR signal modulatory properties that triggered lysosome synthesis and contributed to reversing the adverse effects of amikacin on renal tissues.
Keyphrases
- mass spectrometry
- induced apoptosis
- liquid chromatography
- cell death
- transcription factor
- pet ct
- molecular dynamics simulations
- protein protein
- end stage renal disease
- newly diagnosed
- loop mediated isothermal amplification
- cell cycle arrest
- molecular dynamics
- fatty acid
- gene expression
- chronic kidney disease
- ejection fraction
- metabolic syndrome
- emergency department
- oxidative stress
- prognostic factors
- high performance liquid chromatography
- tandem mass spectrometry
- living cells
- patient reported outcomes
- drug induced
- sensitive detection