Semen Ziziphus jujube Saponins Protects HaCaT Cells against UV Damage and Alleviates the Aging of Caenorhabditis elegans .
Ke-Xin HaoHong XieJian-Guo JiangDawei WangWei ZhuPublished in: ACS omega (2023)
The role of Semen Ziziphus jujube saponins in sedative and hypnosis has attracted much attention. The study aimed to investigate its possible UV damage protection and anti-aging effects. Total saponins (SZR I) and purified saponins (SZR II) were analyzed and compared by infrared spectroscopy and high-performance liquid chromatography (HPLC). The protective effects of SZR I, SZR II, and their three monomers on HaCaT cells damaged by UV were studied, and their anti-aging activities were observed by Caenorhabditis elegans with paraquat-induced oxidative stress. The results showed that SZR I and SZR II differ in chemical composition but both have the same three monomers. The cell survival rate treated with SZR I and SZR II at a concentration of 400 μg/mL increased by 34.45 and 88.98%, respectively, indicating that they could promote the proliferation of UVB-damaged HaCaT cells. Jujuboside A, Jujuboside B, and spinosin from the saponins exhibited similar effects on UVB-damaged HaCaT cells. SZR I and SZR II had little effect on reproductive performance but could delay the senescence caused by heat and oxidative stress of the C. elegans model. These results provide useful data that Semen Z. jujube saponin is a potential natural product with UV damage protection and anti-aging characteristics.
Keyphrases
- induced apoptosis
- oxidative stress
- cell cycle arrest
- high performance liquid chromatography
- endoplasmic reticulum stress
- signaling pathway
- mass spectrometry
- cell death
- ms ms
- simultaneous determination
- risk assessment
- tandem mass spectrometry
- solid phase extraction
- machine learning
- nitric oxide
- big data
- diabetic rats
- cell proliferation
- artificial intelligence
- aqueous solution