Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA.
Byung-Sun ParkHeeju JeonSung-Gil ChiTackhoon KimPublished in: BMC biology (2023)
We identified the variations in target cleavage efficiency, even in optimized sgRNA libraries, that pose a strong bias in phenotype and developed an analysis method that corrects phenotype score by the measured differences in the targeting efficiency among sgRNAs. Collectively, we expect that our new analysis method will more accurately identify genes that confer the phenotype of interest.