A native mass spectrometry platform identifies HOP inhibitors that modulate the HSP90-HOP protein-protein interaction.
Clinton G L VealeMaria Mateos-JiménezMichaelone C VaaltynRonel MüllerMatodzi P MakhubuMahama AlhassanBeatriz G de la TorreFernando AlbericioC L Logan MackayAdrienne Lesley EdkinsDavid J ClarkePublished in: Chemical communications (Cambridge, England) (2021)
Herein we describe a native mass spectromery protein-peptide model as a competent surrogate for the HOP-HSP90 protein-protein interaction (PPI), application of which led to the qualititive identification of two new peptides capable of in vitro PPI disruption. This proof of concept study offers a viable alternative for PPI inhibitor screening.