MsSPL12 is a positive regulator in alfalfa (Medicago sativa L.) salt tolerance.
Shiwen LinJie YangYanrong LiuWanjun ZhangPublished in: Plant cell reports (2024)
ratio and relative water content (RWC) under salt stress treatment, but a reduction in electrolyte leakage (EL), reactive oxygen species (ROS), malondialdehyde (MDA), and proline (Pro) compared to wild type (WT) plants. However, transgenic plants overexpressing MsSPL12-SRDX showed an inhibited plant growth and a reduced salt tolerance. RNA-sequencing and quantitative real-time PCR analyses revealed that MsSPL12 affected the expression of plant abiotic resistance-related genes in multiple physiological pathways. The potential MsSPL12-mediated regulatory pathways based on the differentially expressed genes between the MsSPL12 overexpression transgenics and WT controls were predicted. In summary, our study proves that MsSPL12 is a positive regulator in alfalfa salt tolerance and can be used as a new candidate for manipulation to develop forage crops with enhanced salt tolerance.
Keyphrases
- reactive oxygen species
- transcription factor
- plant growth
- wild type
- real time pcr
- single cell
- poor prognosis
- cell proliferation
- dna damage
- cell death
- high resolution
- climate change
- gene expression
- signaling pathway
- risk assessment
- breast cancer cells
- dna methylation
- arabidopsis thaliana
- combination therapy
- bioinformatics analysis