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Dual base editor catalyzes both cytosine and adenine base conversions in human cells.

Xiaohui ZhangBiyun ZhuLiang ChenLing XieWeishi YuYing WangLinxi LiShuming YinLei YangHandan HuHonghui HanYongmei LiLiren WangGeng ChenXueyun MaHongquan GengWanfeng HuangXiufeng PangZuozhen YangYuxuan WuStefan SiwkoRyo KuritaYukio NakamuraLi YangMingyao LiuDali Li
Published in: Nature biotechnology (2020)
Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax's activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.
Keyphrases
  • genome editing
  • crispr cas