Login / Signup

Integrated omics analysis unveils a DNA damage response to neurogenic injury.

Ali Hashemi GheinaniBryan S SackAlex Bigger-AllenHatim ThakerHussein AttaGeorge LambrinosKyle CostaClaire DoyleMehrnaz Gharaee-KermaniSusan PatalanoMary PiperJustin F CotellessaDijana VitkoHaiying LiManubhai Kadayil PrabhakaranVivian CristofaroJohn FroehlichRichard S LeeWei YangMaryrose P SullivanJill A MacoskaRosalyn M Adam
Published in: bioRxiv : the preprint server for biology (2023)
Employed a multi-omics approach, integrating both transcriptomic and proteomic analyses, to investigate the molecular response in a rat model of spinal cord injury (SCI) and the therapeutic effect of inosine.Discovered multiple regulators of the DNA damage response, including PARP-1, using causal network analysis.Observed decreased markers of DNA damage and PARP activity in inosine-treated tissues, indicating the therapeutic potential of inosine in neurogenic dysfunction.Identified significant alterations in molecular pathways associated with protein synthesis, neuroplasticity, wound healing, and neurotransmitter degradation after SCI, and their modulation by inosine, highlighting its neuroprotective effects beyond DNA damage repair.
Keyphrases
  • dna damage
  • spinal cord injury
  • dna repair
  • dna damage response
  • network analysis
  • oxidative stress
  • single cell
  • spinal cord
  • neuropathic pain
  • wound healing
  • gene expression
  • rna seq
  • label free
  • data analysis