CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma.
Yuehong ShenYuling ChenYuntao LinYicun LiPengfei LiuBiru ZhangYufan WangKing-Chi ChanNai-Ki MakMichael KahnRobert Zhong QiHongyu YangPublished in: Cell death & disease (2023)
In oral squamous cell carcinoma (OSCC), a highly aggressive and frequently lethal malignancy, the role and action mechanism of the microtubule regulatory protein CDK5RAP2 have not been fully understood. Here, we show that CDK5RAP2 is highly expressed in OSCC and its expression correlates with clinical stage and lymph node metastasis of the disease. The expression of CDK5RAP2 is regulated by the Wnt signaling pathway. Depletion of CDK5RAP2 inhibits the tumorigenesis and migration of OSCC cells and alters the OSCC cancer stem (-like) cell (CSC) signature. Notably, suppression of CDK5RAP2 expression disrupts spindle orientation during mitosis. Collectively, these results identify CDK5RAP2 as a potential CSC marker and reveal a mechanism that controls the CSC population in OSCC.
Keyphrases
- cell cycle
- lymph node metastasis
- poor prognosis
- cell proliferation
- stem cells
- papillary thyroid
- signaling pathway
- binding protein
- squamous cell carcinoma
- genome wide
- epithelial mesenchymal transition
- single cell
- pi k akt
- transcription factor
- cell cycle arrest
- risk assessment
- young adults
- climate change
- oxidative stress
- small molecule
- human health
- amino acid