Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish.
Xiangcheng FanJichun HanLijun ZhuZhipeng ChenJiajing LiYue GuFeng WangTao WangYunyun YueJing ShangPublished in: Oxidative medicine and cellular longevity (2020)
Cardiovascular disease is the highest cause of death, and atherosclerosis (AS) is the primary pathogenesis of many cardiovascular diseases. In this study, we aim to investigate the possible pharmaceutical effects of Dendrobium huoshanense C. Z. Tang et S. J. Cheng polysaccharide (DHP) in AS. We fed zebrafish with high-cholesterol diet (HCD) to establish a zebrafish AS model and treated with DHP and observed plaque formation and neutrophil counts under a fluorescence microscope. Next, a parallel flow chamber was utilized to establish low shear stress- (LSS-) induced endothelial cell (EC) dysfunction model. We observed that DHP significantly improved HCD-induced lipid deposition, oxidative stress, and inflammatory response, mainly showing that DHP significantly increased superoxide dismutase (SOD) activity, decreased plaque formation, and decreased neutrophil recruitment and the levels of total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), and reactive oxygen species (ROS). Furthermore, DHP significantly improved LSS-induced oxidative stress and EC dysfunction. Our results indicated that DHP can exert treatment effects on AS, which may attribute to its hypolipidemic, antioxidant, anti-inflammatory activities and improving LSS-induced EC dysfunction. DHP has promising potential for further development as a functional natural medicine source targeted at AS prevention.
Keyphrases
- oxidative stress
- cardiovascular disease
- diabetic rats
- high glucose
- reactive oxygen species
- inflammatory response
- anti inflammatory
- endothelial cells
- low density lipoprotein
- dna damage
- coronary artery disease
- drug induced
- type diabetes
- physical activity
- hydrogen peroxide
- induced apoptosis
- ischemia reperfusion injury
- cell death
- weight loss
- fatty acid
- endoplasmic reticulum stress
- signaling pathway
- vascular endothelial growth factor
- drug delivery
- peripheral blood
- heat shock
- water soluble
- human health