Direct whole-genome sequencing of Plasmodium falciparum specimens from dried erythrocyte spots.

Sidsel NagPoul-Erik KofoedJohan UrsingCamilla Koldbæk LemvighRosa Lundbye AllesøeAmabelia RodriguesChristina Aaby SvendsenJacob Dyring JensenMichael AlifrangisOle LundFrank M Aarestrup

Published in: Malaria journal (2018)

This study describes a simple filter paper based protocol for sampling P. falciparum from malaria patients for subsequent direct WGS, enabling acquisition of samples in remote settings with no access to electricity.

Keyphrases

plasmodium falciparum
end stage renal disease
newly diagnosed
ejection fraction
randomized controlled trial
prognostic factors
ultrasound guided