MiR-2b-3p Downregulated PxTrypsin-9 Expression in the Larval Midgut to Decrease Cry1Ac Susceptibility of the Diamondback Moth, Plutella xylostella (L.).
Jie ZhangMingyou LiuLiang WenYanyan HuaRuonan ZhangShuZhong LiJunaid ZafarRui PangHan-Hong XuXiaoxia XuFengliang JinPublished in: Journal of agricultural and food chemistry (2024)
Crystal (Cry) toxins, produced by Bacillus thuringiensis , are widely used as effective biological pesticides in agricultural production. However, insects always quickly evolve adaptations against Cry toxins within a few generations. In this study, we focused on the Cry1Ac protoxin activated by protease. Our results identified PxTrypsin-9 as a trypsin gene that plays a key role in Cry1Ac virulence in Plutella xylostella larvae. In addition, P. xylostella miR-2b-3p, a member of the micoRNA-2 (miR-2) family, was significantly upregulated by Cry1Ac protoxin and targeted to PxTrypsin-9 downregulated its expression. The mRNA level of PxTrypsin-9, regulated by miR-2b-3p, revealed an increased tolerance of P. xylostella larvae to Cry1Ac at the post-transcriptional level. Considering that miR-2b and trypsin genes are widely distributed in various pest species, our study provides the basis for further investigation of the roles of miRNAs in the regulation of the resistance to Cry1Ac and other insecticides.
Keyphrases
- aedes aegypti
- poor prognosis
- risk assessment
- long non coding rna
- cell proliferation
- genome wide
- gene expression
- pseudomonas aeruginosa
- staphylococcus aureus
- binding protein
- drosophila melanogaster
- transcription factor
- heavy metals
- oxidative stress
- copy number
- antimicrobial resistance
- mass spectrometry
- biofilm formation
- human health
- high resolution
- heat shock protein
- water quality
- solid phase extraction