An integrated toolkit for human microglia functional genomics.

Imdadul HaqJason C NgoNainika RoyRichard L PanNadiya NawsheenRebecca ChiuYa ZhangMasashi FujitaRajesh K SoniXuebing WuDavid A BennettVilas MenonMarta OlahFalak Sher

Published in: Stem cell research & therapy (2024)

Our advanced two-step protocol rapidly and efficiently produces authentic iMG. With features like the CRISPR-ON/OFF system and a comprehensive multi-omic data platform, our toolkit equips researchers for robust microglial functional genomic studies. By facilitating detailed SNV investigation and offering a sustainable cell harvest mechanism, the toolkit heralds significant progress in neurodegenerative disease drug research and therapeutic advancement.

Keyphrases

single cell
inflammatory response
endothelial cells
neuropathic pain
randomized controlled trial
genome wide
lipopolysaccharide induced
cell therapy
electronic health record
copy number
lps induced
induced pluripotent stem cells
stem cells
machine learning
bone marrow
gene expression
case control