C/EBPα Regulates PxTreh 1 and PxTreh 2 Trehalase-Related Bt Resistance in Plutella xylostella (L.).
Jia LiuZheming LiuHaihao MaYaying YuChengjia ZhangWei ZhengYilong ManHang ZhuYong ZhouXi ChenXiaomao ZhouAiping ZengPublished in: Insects (2022)
Trehalase regulates energy metabolism in insects by converting trehalose into two glucose molecules. High amounts of trehalase are critical for insect flight and larval stress resistance. However, whether trehalase participates in the development of pesticide resistance remains unclear. In this study, we explored this phenomenon and the mechanism that underlies the regulation of Trehalase transcription. We found that overexpression of PxTreh 1 and PxTreh 2 induced Bacillus thuringiensis (Bt) resistance in Plutella xylostella . The promoter sequences of PxTreh 1 and PxTreh 2 were also cloned and identified. The dual-luciferase reporter system and RNA interference technology revealed that the expression of PxTreh 1 and PxTreh 2 genes is possibly regulated by the CCAAT enhancer-binding protein (C/EBPα). A yeast one-hybrid experiment confirmed the interaction between C/EBPα and the PxTreh 2 promoter. The findings of this study suggest that C/EBPα mediates the adaptability of P. xylostella to adverse environmental stressors by regulating the expression of trehalase.
Keyphrases
- binding protein
- transcription factor
- poor prognosis
- dna methylation
- gene expression
- risk assessment
- cell proliferation
- type diabetes
- genome wide
- blood pressure
- crispr cas
- metabolic syndrome
- long non coding rna
- skeletal muscle
- drug induced
- blood glucose
- oxidative stress
- climate change
- bacillus subtilis
- glycemic control
- genome wide identification