Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening.
Alexandra J E PelgromClaudia-Nicole MeisrimlerJoyce ElberseThijs KoormanMike BoxemGuido Van den AckervekenPublished in: PloS one (2020)
Plant pathogenic bacteria, fungi and oomycetes secrete effector proteins to manipulate host cell processes to establish a successful infection. Over the last decade the genomes and transcriptomes of many agriculturally important plant pathogens have been sequenced and vast candidate effector repertoires were identified using bioinformatic analyses. Elucidating the contribution of individual effectors to pathogenicity is the next major hurdle. To advance our understanding of the molecular mechanisms underlying lettuce susceptibility to the downy mildew Bremia lactucae, we mapped physical interactions between B. lactucae effectors and lettuce candidate target proteins. Using a lettuce cDNA library-based yeast-two-hybrid system, 61 protein-protein interactions were identified, involving 21 B. lactucae effectors and 46 unique lettuce proteins. The top ten interactors based on the number of independent colonies identified in the Y2H and two interactors that belong to gene families involved in plant immunity, were further characterized. We determined the subcellular localization of the fluorescently tagged lettuce proteins and their interacting effectors. Importantly, relocalization of effectors or their interactors to the nucleus was observed for four protein-pairs upon their co-expression, supporting their interaction in planta.
Keyphrases
- type iii
- regulatory t cells
- dendritic cells
- single cell
- mental health
- physical activity
- poor prognosis
- stem cells
- bone marrow
- genome wide
- copy number
- immune response
- binding protein
- staphylococcus aureus
- mesenchymal stem cells
- small molecule
- cystic fibrosis
- pseudomonas aeruginosa
- long non coding rna
- protein protein
- plant growth