Dual-Modal FexCuySe and Upconversion Nanoparticle Assemblies for Intracellular MicroRNA-21 Detection.
Xiaoqian JiangChanglong HaoHongyu ZhangXiaoling WuLiguang XuMaozhong SunChuanlai XuHua KuangPublished in: ACS applied materials & interfaces (2020)
In situ quantification and imaging of low-level intracellular microRNAs (miRs) are important areas in biosensor research. Herein, DNA-driven FexCuySe@upconversion nanoparticle (UCNP) core@satellite nanostructures were developed to probe microRNA-21 (miR-21). FexCuySe@UCNP probes displayed dual signals: upconversion luminescence (UCL) and magnetic resonance imaging (MRI). In the presence of miR-21, the luminescence signal was restored and the T2 value was significantly increased because of dissociation of UCNPs from the assemblies. There was a good linear relationship between the dual signals and the expression levels of miR-21 in the range of 0.035-31.824 amol/ngRNA. The limit of detection (LOD) was 0.0058 amol/ngRNA for the luminescence intensity and 0.0182 amol/ngRNA for the MRI signal. This method opens a new avenue for intracellular miR-21 detection with high sensitivity and specificity.
Keyphrases
- cell proliferation
- long non coding rna
- magnetic resonance imaging
- energy transfer
- quantum dots
- long noncoding rna
- label free
- poor prognosis
- photodynamic therapy
- contrast enhanced
- loop mediated isothermal amplification
- reactive oxygen species
- computed tomography
- sensitive detection
- high resolution
- gold nanoparticles
- magnetic resonance
- fluorescence imaging
- single molecule
- cell free
- mass spectrometry
- circulating tumor
- light emitting
- structural basis