Myristica lowiana Phytochemicals as Inhibitor of Plasmid Conjugation in Escherichia coli.
Awo Afi KwapongSarah SoaresStephen Ping TeoPaul StapletonSimon GibbonsPublished in: Evidence-based complementary and alternative medicine : eCAM (2020)
Hexane extract and methanol fraction from the stem bark of Myristica lowiana specifically and significantly inhibited the conjugal transfer of the IncW plasmid R7K, a plasmid which harbors ampicillin-, streptomycin-, and spectinomycin-resistant genes. The transfer of this plasmid via the conjugative pilli of Escherichia coli was reduced by 76.5 ± 2.0% and 79.0 ± 1.2% by hexane extract and methanol fraction of M. lowiana, respectively. The hexane extract exhibited significant anti-conjugant activity at a non-cytotoxic concentration of 100 mg/L as assessed against adult human dermal fibroblast cells. The hexane extract and methanol fraction were screened using phytochemical tests, NMR spectroscopy, IR spectroscopy, and high-resolution electrospray ionization mass spectrometry (HRESIMS) and were found to contain terpenoids, sterols, and fatty acids.
Keyphrases
- escherichia coli
- high resolution
- oxidative stress
- mass spectrometry
- anti inflammatory
- crispr cas
- fatty acid
- klebsiella pneumoniae
- biofilm formation
- carbon dioxide
- induced apoptosis
- single molecule
- pseudomonas aeruginosa
- high performance liquid chromatography
- young adults
- electron transfer
- antibiotic resistance genes
- ms ms
- solid phase extraction
- anaerobic digestion