Multiplexed Online Monitoring of Microfluidic Free-Flow Electrophoresis via Mass Spectrometry.
Matthias JenderPedro NovoDominic MaehlerUte MünchbergDirk JanasekErik FreierPublished in: Analytical chemistry (2020)
Free-flow electrophoresis is a tool for the continuous fractionation of electrically charged analytes. In this study, we introduce a novel method to couple microchip-based free-flow electrophoresis with mass spectrometry. The successive connection of multiple microchip outlets to the electrospray ionization source of a mass spectrometer is automated using a multiposition valve. With this novel setup, it is possible to continuously fractionate and collect compounds while simultaneously monitoring the process online with mass spectrometry. The functionality of the method is demonstrated by the successful separation and identification of the biomolecules AMP, ATP, and CoA, which are fundamental for numerous biochemical processes in every organism.
Keyphrases
- mass spectrometry
- capillary electrophoresis
- liquid chromatography
- high resolution
- gas chromatography
- high performance liquid chromatography
- social media
- high throughput
- health information
- tandem mass spectrometry
- single cell
- machine learning
- mitral valve
- deep learning
- healthcare
- atrial fibrillation
- fatty acid
- solid phase extraction
- simultaneous determination
- left ventricular