Highly efficient β-lactamase assay applying poly-dimethylacrylamide-based surface functionalization with β-lactam antibiotics and β-lactamase inhibitors.
Simone RentschlerMax BorgolteAlexander FilbertStefan LauferHans-Peter DeignerPublished in: Lab on a chip (2023)
In recent decades, the rise of β-lactamases has substantially led to the emergence and wide spread of antibiotic resistance posing a serious global health threat. There is growing need for the development of rapid, cost-effective and user-friendly diagnostic assays for the accurate detection of β-lactamases to optimize patient outcomes and prevent the spread of multidrug-resistances. In this article, we present a poly-dimethylacrylamide (PDMA)-based surface functionalization to immobilize β-lactam antibiotics and β-lactamase inhibitors of different subclasses. Immobilization was induced via UV-crosslinking through C,H-insertion reactions. The functional coatings were successfully applied in a highly efficient assay for the determination of recombinant β-lactamases as well as β-lactamases isolated from clinically relevant bacterial strains. Thus, this method describes an innovative approach with several significant benefits for diagnostic applications: the creation of specific detection platforms tailored for β-lactamase activity, the development of high-throughput diagnostic assays and benefits regarding stability and shelf-life. Furthermore, this method is highly adaptable to other surfaces, antibiotics, and analytes, offering far-reaching implications for various biomedical, environmental, and antimicrobial applications.
Keyphrases
- highly efficient
- high throughput
- escherichia coli
- gram negative
- klebsiella pneumoniae
- multidrug resistant
- global health
- loop mediated isothermal amplification
- drug resistant
- single cell
- biofilm formation
- public health
- label free
- high resolution
- high glucose
- climate change
- pseudomonas aeruginosa
- oxidative stress
- solid phase extraction
- sensitive detection
- low cost