Inhibition of the lncRNA Coded within Transglutaminase 2 Gene Impacts Several Relevant Networks in MCF-7 Breast Cancer Cells.
Carlo M BergaminiChiara VischioniGianluca AguiariCarmen GrandiAnna TerrazzanStefano VoliniaNicoletta BianchiCristian TaccioliPublished in: Non-coding RNA (2021)
Long non-coding RNAs are nucleotide molecules that regulate transcription in numerous cellular processes and are related to the occurrence of many diseases, including cancer. In this regard, we recently discovered a polyadenylated long non-coding RNA (named TG2-lncRNA) encoded within the first intron of the Transglutaminase type 2 gene (TGM2), which is related to tumour proliferation in human cancer cell lines. To better characterize this new biological player, we investigated the effects of its suppression in MCF-7 breast cancer cells, using siRNA treatment and RNA-sequencing. In this way, we found modifications in several networks associated to biological functions relevant for tumorigenesis (apoptosis, chronic inflammation, angiogenesis, immunomodulation, cell mobility, and epithelial-mesenchymal transition) that were originally attributed only to Transglutaminase type 2 protein but that could be regulated also by TG2-lncRNA. Moreover, our experiments strongly suggest the ability of TG2-lncRNA to directly interact with important transcription factors, such as RXRα and TP53, paving the way for several regulatory loops that can potentially influence the phenotypic behaviour of MCF-7 cells. These considerations imply the need to further investigate the relative relevance of the TG2 protein itself and/or other gene products as key regulators in the organization of breast cancer program.
Keyphrases
- long non coding rna
- breast cancer cells
- transcription factor
- poor prognosis
- genome wide identification
- epithelial mesenchymal transition
- papillary thyroid
- cell cycle arrest
- copy number
- endothelial cells
- genome wide
- oxidative stress
- celiac disease
- single cell
- induced apoptosis
- signaling pathway
- squamous cell
- endoplasmic reticulum stress
- vascular endothelial growth factor
- risk assessment
- binding protein
- squamous cell carcinoma
- cell death
- cell therapy
- dna methylation
- stem cells
- drug induced
- small molecule
- lymph node metastasis
- wound healing
- combination therapy
- high resolution