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Control of arbuscule development by a transcriptional negative feedback loop in Medicago.

Qiang ZhangShuangshuang WangQiujin XieYuanjun XiaLei LuMingxing WangGang WangSiyu LongYunfei CaiLing XuErtao WangYina Jiang
Published in: Nature communications (2023)
Most terrestrial plants establish a symbiosis with arbuscular mycorrhizal fungi (AMF), which provide them with lipids and sugars in exchange for phosphorus and nitrogen. Nutrient exchange must be dynamically controlled to maintain a mutually beneficial relationship between the two symbiotic partners. The WRI5a and its homologues play a conserved role in lipid supply to AMF. Here, we demonstrate that the AP2/ERF transcription factor MtERM1 binds directly to AW-box and AW-box-like cis-elements in the promoters of MtSTR2 and MtSTR, which are required for host lipid efflux and arbuscule development. The EAR domain-containing transcription factor MtERF12 is also directly activated by MtERM1/MtWRI5a to negatively regulate arbuscule development, and the TOPLESS co-repressor is further recruited by MtERF12 through EAR motif to oppose MtERM1/MtWRI5a function, thereby suppressing arbuscule development. We therefore reveal an ERM1/WRI5a-ERF12-TOPLESS negative feedback loop that enables plants to flexibly control nutrient exchange and ensure a mutually beneficial symbiosis.
Keyphrases
  • transcription factor
  • dna binding
  • genome wide identification
  • gene expression
  • fatty acid
  • signaling pathway
  • oxidative stress
  • hepatitis c virus
  • single cell
  • binding protein