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Elucidating the roles of three β-glucuronosyltransferases (GLCATs) acting on arabinogalactan-proteins using a CRISPR-Cas9 multiplexing approach in Arabidopsis.

Yuan ZhangMichael A HeldAllan M Showalter
Published in: BMC plant biology (2020)
This research provides genetic evidence that GLCAT14A-C function in the transfer of GlcA to AGPs, which in turn play a role in a variety of biochemical and physiological phenotypes including calcium binding by AGPs, seed germination, root hair growth, trichome branching, pollen development, silique development, seed set, and adherent seed coat mucilage accumulation.
Keyphrases
  • crispr cas
  • genome editing
  • transcription factor
  • fluorescent probe
  • copy number
  • living cells