Bioassay-Guided Isolation of an Abetiane-Type Diterpenoid from Prunella vulgaris That Protects against Concanavalin A-Induced Autoimmune Hepatitis.
Yunfang CaoXiaosheng TanAli Adhab Al ChnaniPengkun LiZhengyi ShiHao FengLingjuan SunQiangbing XiaXi YangYulin DuanYi GuoGang ChenChangxing QiYong-Hui ZhangPublished in: Journal of natural products (2021)
Prunella vulgaris is a widely used edible Chinese medicinal plant. In the present study, two new abietane-type diterpenoids, abietoquinones A (1) and B (2), were isolated from this plant by an immunosuppressive bioassay-guided isolation procedure. Their structures were elucidated unambiguously by NMR spectroscopic analysis, single-crystal X-ray crystallography, and electronic circular dichroism calculations. Compounds 1 and 2 bear a cyclohex-2-ene-1,4-dione moiety, which is uncommon among abietane diterpenes. Also, abietoquinone A (1) suppressed murine splenocyte proliferation and decreased the production of proinflammatory cytokines induced by concanavalin A (Con A) in vitro. In Con A-challenged mice, preinjection with 1 significantly ameliorated liver injury. Additionally, abietoquinone A (1) exhibited inhibitory activities against the proliferation of murine splenocytes and human T cells induced by anti-CD3/anti-CD28 monoclonal antibodies (mAbs).
Keyphrases
- drug induced
- liver injury
- high resolution
- signaling pathway
- endothelial cells
- high glucose
- magnetic resonance
- solid state
- molecular docking
- nk cells
- multiple sclerosis
- density functional theory
- minimally invasive
- cell wall
- pluripotent stem cells
- diabetic rats
- molecular dynamics simulations
- type diabetes
- mass spectrometry
- wild type
- data analysis