Genome editing with CRISPR/Cas9 in Pinus radiata (D. Don).
Charleson PoovaiahLorelle PhillipsBarbara GeddesCathie ReevesMathias SorieulGlenn ThorlbyPublished in: BMC plant biology (2021)
We have demonstrated the generation of biallelic and monoallelic INDELs in the coniferous tree P. radiata with the CRISPR/Cas9 system using plasmid expressed Cas9 gRNA and RNPs respectively. This opens the opportunity to apply genome editing in conifers to rapidly modify key traits of interest.