Mid-pregnancy maternal blood nitric oxide-related gene and miRNA expression are associated with preterm birth.
Tracy A ManuckLauren A EavesJulia E RagerRebecca C FryPublished in: Epigenomics (2021)
Aim: The nitric oxide (NO) pathway modulates inflammation and may influence birth timing. Patients & methods: Case-control analysis of 136 pregnant women with RNA obtained <28 weeks; n = 212 mRNAs and n = 108 miRNAs in the NO pathway were evaluated. NO-pathway mRNA and miRNA transcript counts in women delivering preterm versus at term were compared, miRNA-mRNA expression levels correlated and prediction models generated. Results: Fourteen genes were differentially expressed in women delivering <37 weeks; 13/14 were also differentially expressed in those delivering <34 weeks (q <0.10) versus term births. Multiple miRNA-mRNA pairs were correlated. Models with gene expression better predicted prematurity than models with only clinical or nongenomic predictors. Conclusion: Maternal blood NO pathway-related mRNA and miRNA expression is associated with prematurity.
Keyphrases
- gestational age
- preterm birth
- birth weight
- low birth weight
- nitric oxide
- pregnancy outcomes
- gene expression
- binding protein
- preterm infants
- poor prognosis
- polycystic ovary syndrome
- end stage renal disease
- pregnant women
- genome wide
- case control
- newly diagnosed
- dna methylation
- hydrogen peroxide
- oxidative stress
- nitric oxide synthase
- ejection fraction
- prognostic factors
- metabolic syndrome
- type diabetes
- rna seq
- weight gain
- copy number
- long non coding rna