Sodium Polyanethol Sulfonate Modulates Natural Transformation of SigH-Expressing Staphylococcus aureus.
Le Thuy Thi NguyenAya J TakemuraRyosuke L OhniwaShinji SaitoKazuya MorikawaPublished in: Current microbiology (2017)
Expression of genes required for natural genetic competence in Staphylococcus aureus is controlled by an alternative transcription sigma factor, SigH. However, even in the SigH-expressing cells, the DNA transformation efficiency varies depending on culture conditions. We report here that cells grown in the competence-inducing medium (CS2 medium) exhibit enlarged morphology with disintegrated cell walls. Notably, an autolysis inhibitor, Sodium Polyanethol Sulfonate (SPS), facilitated transformation in CS2 medium in a dose-dependent manner, suggesting the involvement of the cell wall metabolism in transformation. However, the transformation efficiency of cells grown in TSB was not improved by physical or enzymatic damage on the cell walls.
Keyphrases
- induced apoptosis
- staphylococcus aureus
- cell cycle arrest
- single cell
- cell wall
- oxidative stress
- genome wide
- gene expression
- signaling pathway
- stem cells
- cell death
- physical activity
- poor prognosis
- cystic fibrosis
- nitric oxide
- biofilm formation
- pseudomonas aeruginosa
- binding protein
- single molecule
- hydrogen peroxide
- methicillin resistant staphylococcus aureus
- copy number
- cell free
- circulating tumor cells