Effects of pre-treatments on bulk stable isotope ratios in fish samples: A cautionary note for studies comparisons.

The chemical properties of compounds (e.g. urea, lipids) combined with the polarity of solutions (e.g. water, solvents) explained a large part of these observations. This study highlights that pre-treatments need to be considered especially when comparing carbon and nitrogen stable isotope ratios between studies. The results of this study provide a call to all stable isotope researchers to make a concerted effort to standardise pre-treatment methods. This is crucial as global reviews are becoming increasingly more informative.