HiChIP: efficient and sensitive analysis of protein-directed genome architecture.

Maxwell R MumbachAdam J RubinRyan A FlynnChao DaiPaul A KhavariWilliam J GreenleafHoward Y Chang

Published in: Nature methods (2016)

Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.

Keyphrases

genome wide
molecular dynamics simulations
crystal structure
dna methylation
protein protein
gene expression
dna damage
amino acid
binding protein
transcription factor
oxidative stress
pet imaging