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CRISPR-Guided Proximity Labeling of RNA-Protein Interactions.

Mingxing LuZuowei WangYixiu WangBingbing Ren
Published in: Genes (2022)
Proximity labeling employs modified biotin ligases or peroxidases that produce reactive radicals to covalently label proximate proteins with biotin in living cells. The resulting biotinylated proteins can then be isolated and identified. A combination of programmable DNA targeting and proximity labeling that maps proteomic landscape at DNA elements with dCas9-APEX2 has been established in living cells. However, defining interactome at RNA elements has lagged behind. In combination with RNA-targeting CRISPR-Cas13, proximity labeling can also be used to identify proteins that interact with specific RNA elements in living cells. From this viewpoint, we briefly summarize the latest advances in CRISPR-guided proximity labeling in studying RNA-protein interactions, and we propose applying the most recent engineered proximity-labeling enzymes to study RNA-centric interactions in the future.
Keyphrases
  • living cells
  • crispr cas
  • nucleic acid
  • single molecule
  • fluorescent probe
  • genome editing
  • genome wide
  • cell free
  • circulating tumor
  • cancer therapy
  • gene expression
  • amino acid
  • small molecule