Inkjet-Printed Phospholipid Bilayers on Titanium Oxide Surfaces: Towards Functional Membrane Biointerfaces.
Sigalit MekerOded HaleviHokyun ChinTun Naw SutJoshua A JackmanEe-Lin TanMichael G PotrozNam-Joon ChoPublished in: Membranes (2022)
Functional biointerfaces hold broad significance for designing cell-responsive medical implants and sensor devices. Solid-supported phospholipid bilayers are a promising class of biological materials to build bioinspired thin-film coatings, as they can facilitate interactions with cell membranes. However, it remains challenging to fabricate lipid bilayers on medically relevant materials such as titanium oxide surfaces. There are also limitations in existing bilayer printing capabilities since most approaches are restricted to either deposition alone or to fixed microarray patterning. By combining advances in lipid surface chemistry and on-demand inkjet printing, we demonstrate the direct deposition and patterning of covalently tethered lipid bilayer membranes on titanium oxide surfaces, in ambient conditions and without any surface pretreatment process. The deposition conditions were evaluated by quartz crystal microbalance-dissipation (QCM-D) measurements, with corresponding resonance frequency (Δf) and energy dissipation (ΔD) shifts of around -25 Hz and <1 × 10 -6 , respectively, that indicated successful bilayer printing. The resulting printed phospholipid bilayers are stable in air and do not collapse following dehydration; through rehydration, the bilayers regain their functional properties, such as lateral mobility (>1 µm 2 /s diffusion coefficient), according to fluorescence recovery after photobleaching (FRAP) measurements. By taking advantage of the lipid bilayer patterned architectures and the unique features of titanium oxide's photoactivity, we further show how patterned cell culture arrays can be fabricated. Looking forward, this work presents new capabilities to achieve stable lipid bilayer patterns that can potentially be translated into implantable biomedical devices.
Keyphrases
- fatty acid
- molecular dynamics simulations
- single cell
- biofilm formation
- healthcare
- stem cells
- computed tomography
- minimally invasive
- magnetic resonance
- cystic fibrosis
- mesenchymal stem cells
- drug delivery
- pseudomonas aeruginosa
- low cost
- diffusion weighted imaging
- solid state
- candida albicans
- gastric bypass
- oxide nanoparticles
- roux en y gastric bypass