Trapa bispinosa Roxb. Pericarp Extract Exerts 5α-Reductase Inhibitory Activity in Castrated Benign Prostatic Hyperplasia Model Mice.
Takashi FujitaTomoko AoyamaTomohiro UemuraShouko TakeshitaTakuto YamasakiHiroko HeijouKoji MorimotoPublished in: International journal of molecular sciences (2023)
Trapa bispinosa Roxb. pericarp extract (TBE) has a polyphenol-rich composition and exhibits potent antioxidant and anti-glycation activities in vitro. In the present study, we investigated the inhibitory effects of TBE on 5α-reductase in vitro using LNCaP cells and in vivo using a mouse model of castrated benign prostatic hyperplasia. TBE showed concentration-dependent inhibitory effects in the 5α-reductase (5αR) activity assay. In a reporter assay using AR-Luc/LNCaP cells, TBE inhibited the activity induced by testosterone, but not that induced by dihydrotestosterone. TBE also suppressed prostate cell proliferation, prostate-specific antigens, and transmembrane protease serine 2 expression in a castrated benign prostatic hyperplasia mouse model. In addition, ellagic acid, but not gallic acid, decreased 5αR and AR-Luc activities. Together, these results suggest a potential role for TBE in benign prostatic hyperplasia through inhibition of 5αR.
Keyphrases
- benign prostatic hyperplasia
- lower urinary tract symptoms
- mouse model
- induced apoptosis
- oxidative stress
- cell proliferation
- anti inflammatory
- cell cycle arrest
- high throughput
- poor prognosis
- prostate cancer
- immune response
- crispr cas
- dendritic cells
- high fat diet induced
- type diabetes
- mass spectrometry
- metabolic syndrome
- insulin resistance
- single molecule
- smoking cessation