The Arabidopsis J-Protein AtDjC5 Facilitates Thermotolerance Likely by Aiding in the ER Stress Response.
Ting-Ting ShenLin WangChun-Huan ShangYi-Cai ZhenYu-Lu FangLi-Li WeiTing ZhouJiao-Teng BaiBing LiPublished in: International journal of molecular sciences (2022)
AtDjC5 belongs to the J-protein family in Arabidopsis thaliana . Its biological functions remain unclear. In this study, we examined the roles of AtDjC5 in resisting heat stress using reverse genetic analysis. After the seedlings were exposed directly to 44 °C for 90 min, AtDjC5 knockout seedlings displayed decreases in the survival rate, membrane system stability, and cell vitality compared to WT seedlings, indicating that AtDjC5 is involved in plant basal thermotolerance. The AtDjC5 knockout seedlings pre-exposed to 37 °C for 30 min exhibited decreases in the survival rate and total chlorophyll contents and increased cell death when they were subsequently exposed to 45 °C compared to the WT seedlings, indicating that AtDjC5 plays an important role in plant acquired thermotolerance. AtDjC5 was found to localize to the endoplasmic reticulum. The expression of the AtDjC5 gene was induced by heat and TM (an ER stress inducer) treatment. Furthermore, we found that the knockout of AtDjC5 inhibited ER stress-induced autophagy and the expression of ER stress-related genes. Taken together, these results suggest that AtDjC5 facilitates thermotolerance, likely by aiding in the ER stress response.
Keyphrases
- arabidopsis thaliana
- endoplasmic reticulum
- heat stress
- heat shock
- cell death
- stress induced
- poor prognosis
- binding protein
- estrogen receptor
- breast cancer cells
- protein protein
- oxidative stress
- copy number
- amino acid
- genome wide
- single cell
- plant growth
- cell wall
- heat shock protein
- long non coding rna
- free survival
- small molecule
- combination therapy
- dna methylation
- replacement therapy