Ascorbic acid attenuates gasoline-induced testicular toxicity, sperm quality deterioration, and testosterone imbalance in rats.
Ogechukwu E EzimJoy NyecheChisom E NebeolisaChuka D BelonwuSunny O AbarikwuPublished in: Toxicology and industrial health (2024)
The present study evaluated the protective effect of ascorbic acid (ASCB) against gasoline fumes (PET) induced testicular oxidative stress, sperm toxicity, and testosterone imbalance in Wistar rats. Twenty-four (24) male albino rats (75 ± 16 g) were randomized into three experimental groups ( N = 8). The control group: received normal saline, PET group: exposed to PET 6 h daily by inhalation in an exposure chamber and PET + 200 mg ASCB/kg body weight group: exposed to PET 6 h daily by inhalation and administered ASCB per os . Treatment of ASCB and PET exposure was done thrice and five times weekly for a period of 10 weeks respectively. ASCB co-treatment prevented PET-induced increases in the oxidative stress markers (glutathione, glutathione S -transferase, superoxide dismutase, catalase, hydrogen peroxide generation, nitric oxide, and lipid peroxidation) and serum testosterone concentration ( p < .05). Sperm quality was low and those with damaged heads and tails increased alongside histological injuries in the PET-exposed rats, which were also minimized with ASCB administration. ASCB protected against PET-induced oxidative stress, sperm, and testis damage in rats.
Keyphrases
- pet ct
- oxidative stress
- positron emission tomography
- hydrogen peroxide
- computed tomography
- nitric oxide
- pet imaging
- diabetic rats
- body weight
- replacement therapy
- physical activity
- dna damage
- randomized controlled trial
- clinical trial
- high glucose
- induced apoptosis
- signaling pathway
- double blind
- nitric oxide synthase
- preterm birth