Decellularized bovine ovarian niche restored the function of cumulus and endothelial cells.

Farhad AmjadiRahim BeheshtiFatemeh Sokouti NasimiAyla HassaniReza ShiraziAmin TamadonReza RahbarghaziMahdi Mahdipour

Published in: BMC research notes (2022)

Bright-field imaging and DAPI staining revealed the lack of nuclei with naive matrix structure in ovarian tissue subjected to decellularization protocol. SEM imaging revealed a normal matrix in decellularized ovaries. LDH assay showed a lack of cytotoxicity for CCs after 7-days compared to the control group. Immunohistochemistry staining showed both CD31- and estrogen-positive cells in CCs + HUVECs compared to the CCs group. CD31 cells appeared with flattened morphology aligned with matrix fibers. The existence of estrogen and CD31 positive cells showed the efficiency of decellularized ovarian tissue to restore cellular function and activity.

Keyphrases

induced apoptosis
cell cycle arrest
high resolution
extracellular matrix
randomized controlled trial
endoplasmic reticulum stress
high throughput
single cell
cell death
hiv infected
estrogen receptor
photodynamic therapy
tissue engineering
vascular endothelial growth factor
flow cytometry
fluorescence imaging