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A ligation-driven CRISPR-Cas biosensing platform for non-nucleic acid target detections.

Jiali ZhaoZhen TanLiu WangChunyang LeiZhou Nie
Published in: Chemical communications (Cambridge, England) (2021)
Herein, we describe a CRISPR-Cas12a sensing platform activated by a DNA ligation reaction for the sensitive detection of non-nucleic acid targets, including NAD+, ATP and polynucleotide kinase (PNK). In this design, the DNA ligation reaction triggered by these biomolecules generates DNA duplexes, which can activate the nuclease activity of Cas12a to produce amplified fluorescence signals. As a result, this work provides an alternative strategy to expand the applicability of the CRISPR-Cas system into the detection of non-nucleic acid biomolecules.
Keyphrases
  • nucleic acid
  • crispr cas
  • genome editing
  • sensitive detection
  • loop mediated isothermal amplification
  • high throughput
  • quantum dots
  • label free
  • single molecule
  • circulating tumor
  • cell free
  • electron transfer
  • dna binding