Antimicrobial activity of hydralazine against methicillin-resistant and methicillin-susceptible Staphylococcus aureus .
Francisca Bsa do NascimentoLívia Gurgel do Amaral Valente SáJoão Batista de Andrade NetoLisandra Juvêncio da SilvaDaniel Sampaio RodriguesVitória Pessoa de Farias CabralAmanda Dias BarbosaLara Elloyse Almeida MoreiraCamille R Braga VasconcelosBruno Coêlho CavalcantiMaria E França RiosJacilene SilvaEmanuelle Machado MarinhoHélcio Silva Dos SantosJacó Rl de MesquitaMarina Duarte Pinto LoboManoel Odorico DE-MoraesHélio Vitoriano Nobre JúniorCecília Rocha da SilvaPublished in: Future microbiology (2024)
Background: Staphylococcus aureus is a human pathogen responsible for high mortality rates. The development of new antimicrobials is urgent. Materials & methods: The authors evaluated the activity of hydralazine along with its synergism with other drugs and action on biofilms. With regard to action mechanisms, the authors evaluated cell viability, DNA damage and molecular docking. Results: MIC and minimum bactericidal concentration values ranged from 128 to 2048 μg/ml. There was synergism with oxacillin (50%) and vancomycin (25%). Hydralazine reduced the viability of biofilms by 50%. After exposure to hydralazine 2× MIC, 58.78% of the cells were unviable, 62.07% were TUNEL positive and 27.03% presented damage in the comet assay (p < 0.05). Hydralazine showed affinity for DNA gyrase and TyrRS. Conclusion: Hydralazine is a potential antibacterial.
Keyphrases
- staphylococcus aureus
- molecular docking
- methicillin resistant staphylococcus aureus
- dna damage
- biofilm formation
- candida albicans
- oxidative stress
- induced apoptosis
- endothelial cells
- cardiovascular events
- risk factors
- cell cycle arrest
- high throughput
- dna repair
- type diabetes
- single molecule
- cell death
- mass spectrometry
- climate change
- human health
- risk assessment
- pluripotent stem cells
- drug induced