Thiazole orange-carboplatin triplex-forming oligonucleotide (TFO) combination probes enhance targeted DNA crosslinking.
Joseph HennessyPiotr KlimkowskiDaniel SingletonAlex GibneyMalou CocheNicholas P FarrellAfaf H El-SagheerTom BrownAndrew KellettPublished in: RSC medicinal chemistry (2023)
We report a new class of carboplatin-TFO hybrid that incorporates a bifunctional alkyne-amine nucleobase monomer called AP-C3-dT that enables dual 'click' platinum(ii) drug conjugation and thiazole orange fluorophore coupling. Thiazole orange enhances the binding of Pt(ii)-TFO hybrids and provides an intrinsic method for monitoring triplex formation. These hybrid constructs possess increased stabilisation and crosslinking properties in comparison to earlier Pt(ii)-TFOs, and demonstrate sequence-specific binding at neutral pH.
Keyphrases
- single molecule
- phase ii study
- transcription factor
- dna binding
- circulating tumor
- emergency department
- squamous cell carcinoma
- binding protein
- radiation therapy
- room temperature
- living cells
- highly efficient
- molecularly imprinted
- locally advanced
- electronic health record
- liquid chromatography
- simultaneous determination
- ionic liquid