Methotrexate Alters the Expression of microRNA in Fibroblast-like Synovial Cells in Rheumatoid Arthritis.
Naoki IwamotoKaori FurukawaYushiro EndoToshimasa ShimizuRemi SumiyoshiMasataka UmedaTomohiro KogaShin-Ya KawashiriTakashi IgawaKunihiro IchinoseMami TamaiTomoki OriguchiAtsushi KawakamiPublished in: International journal of molecular sciences (2021)
We aimed to investigate the effect of methotrexate (MTX) on microRNA modulation in rheumatoid arthritis fibroblast-like synovial cells (RA-FLS). RA-FLS were treated with MTX for 48 h. We then performed miRNA array analysis to investigate differentially expressed miRNAs. Transfection with miR-877-3p precursor and inhibitor were used to investigate the functional role of miR-877-3p in RA-FLS. Gene ontology analysis was used to investigate the cellular processes involving miR-877-3p. The production of cytokines/chemokines was screened by multiplex cytokine/chemokine bead assay and confirmed by ELISA and quantitative real-time PCR. The migratory and proliferative activities of RA-FLS were analyzed by wound healing assay and MKI-67 expression. MTX treatment altered the expression of 13 miRNAs (seven were upregulated and six were downregulated). Among them, quantitative real-time PCR confirmed that miR-877-3p was upregulated in response to MTX (1.79 ± 0.46-fold, p < 0.05). The possible target genes of miR-877-3p in RA-FLS revealed by the microarray analysis were correlated with biological processes. The overexpression of miR-877-3p decreased the production of GM-CSF and CCL3, and the overexpression of miR-877-3p inhibited migratory and proliferative activity. MTX altered the miR-877-3p expression on RA-FLS, and this alteration of miR-877-3p attenuated the abundant production of cytokines/chemokines and proliferative property of RA-FLS.
Keyphrases
- rheumatoid arthritis
- disease activity
- real time pcr
- poor prognosis
- ankylosing spondylitis
- interstitial lung disease
- high throughput
- induced apoptosis
- high resolution
- wound healing
- systemic lupus erythematosus
- cell proliferation
- long non coding rna
- oxidative stress
- systemic sclerosis
- transcription factor
- cell cycle arrest
- copy number
- low dose
- gene expression
- genome wide identification
- idiopathic pulmonary fibrosis