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Elucidation of Olive Oil Oxidation Mechanisms by Analysis of Triacylglycerol Hydroperoxide Isomers Using LC-MS/MS.

Hayato TakahashiShunji KatoNaoki ShimizuYurika OtokiJunya ItoMasayoshi SakainoTakashi SanoJun ImagiKiyotaka Nakagawa
Published in: Molecules (Basel, Switzerland) (2022)
Despite the importance of the insight about the oxidation mechanisms (i.e., radical and singlet oxygen ( 1 O 2 ) oxidation) in extra virgin olive oil (EVOO), the elucidation has been difficult due to its various triacylglycerol molecular species and complex matrix. This study tried to evaluate the mechanisms responsible for EVOO oxidation in our daily use by quantitative determination of triacylglycerol hydroperoxide (TGOOH) isomers using LC-MS/MS. The standards of dioleoyl-(hydroperoxy octadecadienoyl)-triacylglycerol and dioleoyl-(hydroperoxy octadecamonoenoyl)-triacylglycerol, which are the predominant TGOOHs contained in EVOO, were prepared. Subsequently, fresh, thermal-, and photo-oxidized EVOO were analyzed. The obtained results mostly agreed with the previously reported characteristics of the radical and 1 O 2 oxidation of linoleic acid and oleic acid. This suggests that the methods described in this paper should be valuable in understanding how different factors that determine the quality of EVOO (e.g., olive species, cultivation area, cultivation timing, and extraction methods) contribute to its oxidative stability.
Keyphrases
  • hydrogen peroxide
  • electron transfer
  • visible light
  • fatty acid
  • genetic diversity
  • solid phase extraction
  • simultaneous determination
  • low density lipoprotein