Synthesis, characterization, and evaluation of curcumin-loaded endodontic reparative material.
Mahdieh AlipourSadaf FadakarMarziyeh AghazadehRoya SalehiHossein Samadi KafilLeila RoshangarEnsieh MousaviZahra AghazadehPublished in: Journal of biochemical and molecular toxicology (2021)
Curcumin (CUR) is an ancient therapeutic agent with remarkable antimicrobial and anti-inflammatory properties. The purpose of the current study was to synthesize and evaluate a curcumin-based reparative endodontic material to reduce infection and inflammation besides the induction of mineralization during the healing of the dentin-pulp complex. Poly-ɛ-caprolactone (PCL)/gelatin (Gel)/CUR scaffold was synthesized and assessed by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermo-gravimetric analysis (TGA). Agar diffusion test was performed against E. coli, A. baumannii, P. aeruginosa, S. aureus, E. faecalis, and S. mutans. Moreover, proliferative, antioxidative, anti-inflammatory, and calcification properties of these scaffolds on human dental pulp stem cells (hDPSCs) were evaluated. The results showed that PCL/Gel/CUR scaffold had antibacterial effects. Also, these CUR-based scaffolds had significant inhibitory effects on the expression of tumor necrosis factor α and DCF from inflamed hDPSCs (p < 0.05). Moreover, the induction of mineralization in hDPSCs significantly increased after seeding on CUR-based scaffolds (p < 0.05). Based on these findings, the investigated CUR-loaded material was fabricated successfully and provided an appropriate structure for the attachment and proliferation of hDPSCs. It was found that these scaffolds had antimicrobial, antioxidant, and anti-inflammatory characteristics and could induce mineralization in hDPSCs, which is essential for healing and repairing the injured dentin-pulp complex.
Keyphrases
- anti inflammatory
- tissue engineering
- electron microscopy
- stem cells
- staphylococcus aureus
- wound healing
- drug delivery
- endothelial cells
- oxidative stress
- cancer therapy
- poor prognosis
- rheumatoid arthritis
- high resolution
- signaling pathway
- biofilm formation
- induced pluripotent stem cells
- pseudomonas aeruginosa
- atomic force microscopy
- mesenchymal stem cells