To clarify the chemical basis of the total alkaloids of Uncaria rhynchophylla , HPLC-VWD chromatogram of total alkaloids was established. Under its guidance, modern chromatographic and spectroscopic techniques were used to track, isolate and identify the representative principal components. As a result, one new monoterpenoid indole alkaloid, 3 S ,15 S - N 4-methoxymethyl-geissoschizine methyl ether (1), together with 20 known alkaloids ( 2-21 ), and 5 other known compounds ( 22-26 ) were obtained. Meanwhile, sixteen characteristic peaks were identified from the total alkaloids using HPLC analysis. Then, the anti-neuroinflammatory effect of compounds 1 - 21 was assessed through inhibiting nitric ---oxide (NO) production in lipopolysaccharide (LPS)-induced BV-2 microglial cells. Among them, compounds 1 , 3 , 7 , 8 , 11 , 12 , 19 and 21 showed potent inhibitory activities with IC 50 values of 5.87-76.78 μ M.
Keyphrases
- lps induced
- inflammatory response
- simultaneous determination
- nitric oxide
- ms ms
- lipopolysaccharide induced
- induced apoptosis
- signaling pathway
- mass spectrometry
- molecular docking
- solid phase extraction
- high resolution
- immune response
- cell proliferation
- cell death
- cross sectional
- liquid chromatography
- spinal cord injury