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Distinct progenitor lineages contribute to the heterogeneity of plasmacytoid dendritic cells.

Patrick Fernandes RodriguesLlucia Alberti-ServeraAnna EreminGary E Grajales-ReyesRobert IvanekRoxane Tussiwand
Published in: Nature immunology (2018)
Plasmacytoid dendritic cells (pDCs) are an immune subset devoted to the production of high amounts of type 1 interferons in response to viral infections. Whereas conventional dendritic cells (cDCs) originate mostly from a common dendritic cell progenitor (CDP), pDCs have been shown to develop from both CDPs and common lymphoid progenitors. Here, we found that pDCs developed predominantly from IL-7R+ lymphoid progenitor cells. Expression of SiglecH and Ly6D defined pDC lineage commitment along the lymphoid branch. Transcriptional characterization of SiglecH+Ly6D+ precursors indicated that pDC development requires high expression of the transcription factor IRF8, whereas pDC identity relies on TCF4. RNA sequencing of IL-7R+ lymphoid and CDP-derived pDCs mirrored the heterogeneity of mature pDCs observed in single-cell analysis. Both mature pDC subsets are able to secrete type 1 interferons, but only myeloid-derived pDCs share with cDCs their ability to process and present antigen.
Keyphrases
  • dendritic cells
  • single cell
  • rna seq
  • transcription factor
  • regulatory t cells
  • immune response
  • poor prognosis
  • high throughput
  • sars cov
  • gene expression
  • cell fate
  • bone marrow
  • acute myeloid leukemia
  • long non coding rna