NF-κB, iNOS, IL-6, and collagen 1 and 5 expression in healthy and keratoconus corneal fibroblasts after 0.1% riboflavin UV-A illumination.
Tim BergerNóra SzentmáryLorenz LattaBerthold SeitzTanja StachonPublished in: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie (2021)
Our study confirmed altered gene expression in untreated KC-HCFs compared to untreated HCFs. Riboflavin UV-A illumination affected gene expression only in HCFs. Increased IL-6 concentration in the cell culture supernatant and cell lysate indicate a secondary inflammatory response of HCFs and KC-HCFs to riboflavin UV-A illumination.
Keyphrases
- gene expression
- inflammatory response
- dna methylation
- lps induced
- poor prognosis
- signaling pathway
- single cell
- wound healing
- cell therapy
- lipopolysaccharide induced
- oxidative stress
- cell free
- stem cells
- nuclear factor
- extracellular matrix
- immune response
- mesenchymal stem cells
- nitric oxide synthase
- tissue engineering
- nitric oxide