Expression, purification, and characterization of self-assembly virus-like particles of capsid protein L1 HPV 52 in Pichia pastoris GS115.
Chindy Nur RosmeitaSri BudiartiApon Zaenal MustopaEla NoviantiSri SwasthikawatiSheila ChairunnisaAi HertatiMaritsa NurfatwaNurlaili EkawatiNurhasni HasanPublished in: Journal, genetic engineering & biotechnology (2023)
The recombinant L1 HPV 52 protein is successfully expressed in P. pastoris within a size range of approximately 55 kDa and demonstrated favorable stability. The L1 protein expressed in Pichia pastoris successful self-assembled of HPV VLPs, thereby establishing their potential efficacy as a prophylactic vaccine.