Simplified in vitro engineering of neuromuscular junctions between rat embryonic motoneurons and immortalized human skeletal muscle cells.

Jasdeep SainiAlessandro FaroniMarwah Abd Al SamidAdam J ReidAdam P LightfootKamel MamchaouiVincent MoulyGillian Butler-BrowneJamie S McPheeHans DegensNasser Al-Shanti

Published in: Stem cells and cloning : advances and applications (2019)

This coculture system provides a tool to study vital features of NMJ formation, regulation, maintenance, and repair, as well as a model platform to explore neuromuscular diseases and disorders affecting NMJs.

Keyphrases

skeletal muscle
induced apoptosis
endothelial cells
cell cycle arrest
oxidative stress
insulin resistance
single molecule
high throughput
type diabetes
cell death
pluripotent stem cells
signaling pathway