An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in peach reproductive tissues.
Monica CantonSilvia FarinatiCristian ForestanJustin JosephClaudio BonghiSerena VarottoPublished in: Plant methods (2022)
We present general strategies to optimize ChIP protocols for buds and mesocarp tissues of peach and analyze the correlation between gene expression and chromatin mark enrichment/depletion. The procedures proposed may be useful to evaluate any involvement of histone modifications in the regulation of gene expression during bud dormancy progression and core ripening in fruits.