Development and validation of a solid-phase extraction method coupled with LC-MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat.
Weijia ZhengA M Abd El-AtySeong-Kwan KimJeong-Min ChoiDa-Hee ParkKyung-Hee YooYoung-Sun KangJong-Sup JeonAhmet HacımüftüoğluJae-Han ShimHo-Chul ShinPublished in: Biomedical chromatography : BMC (2019)
The present study was carried out to determine 16 antibiotics belonging to seven different groups (tetracyclines, sulfonamides, penicillins, fluoroquinolones, macrolides, lincosamides and trimethoprims) in duck meat. A solid-phase extraction method based on Oasis HLB cartridges coupled with liquid chromatography-electrospray ionization tandem mass spectrometry was developed. Solutions of 0.1 m ethylenediaminetetraacetic acid disodium salt and 2% trifluoroacetic acid were used for the preliminary extraction of the target antibiotics from duck meat and n-hexane was used for purification prior to solid-phase extraction. Mobile phases composed of 0.1% trifluoroacetic acid in distilled water (solvent A) and 0.1% trifluoroacetic acid in methanol (solvent B), combined with a reversed-phase C18 analytical column, provided the optimal separation and signal intensity. The linearity of the method was assessed using six concentrations (5, 10, 20, 30, 40, and 50 μg/kg), and the recoveries, which were calculated at three spiking concentrations (5, 10 and 20 μg/kg), were in the range 69.8-103.3% with relative standard deviations (RSDs) ≤ 6.9% for the 16 tested antibiotics. Matrix effects ranging from -47.2 to -13.5% were observed for all the analytes, and the limits of quantitation (LOQ), which ranged from 4.93 to 26.21 μg/kg, were much lower than the maximum residue limits (MRLs) set by various regulatory authorities. Ten samples from a market were tested, and none of the target analytes were detected. Thus, a simple and versatile protocol has been developed to detect and quantify 16 antibiotics in duck meat samples.
Keyphrases
- solid phase extraction
- liquid chromatography
- tandem mass spectrometry
- simultaneous determination
- ultra high performance liquid chromatography
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- mass spectrometry
- molecularly imprinted
- high resolution mass spectrometry
- gas chromatography
- gas chromatography mass spectrometry
- randomized controlled trial
- ionic liquid
- high resolution
- transcription factor
- ms ms
- health insurance