Identification of Regulatory Elements in Primary Sensory Neurons Involved in Trauma-Induced Neuropathic Pain.
Kimberly E StephensCedric MooreDavid A VinsonBryan E WhiteZachary RenfroWeiqiang ZhouZhicheng JiHongkai JiHeng ZhuYun GuanSean D TavernaPublished in: Molecular neurobiology (2023)
Chronic pain is a significant public health issue that is often refractory to existing therapies. Here we use a multiomic approach to identify cis-regulatory elements that show differential chromatin accessibility and reveal transcription factor (TF) binding motifs with functional regulation in the rat dorsal root ganglion (DRG), which contain cell bodies of primary sensory neurons, after nerve injury. We integrated RNA-seq to understand how differential chromatin accessibility after nerve injury may influence gene expression. Using TF protein arrays and chromatin immunoprecipitation-qPCR, we confirmed C/EBPγ binding to a differentially accessible sequence and used RNA-seq to identify processes in which C/EBPγ plays an important role. Our findings offer insights into TF motifs that are associated with chronic pain. These data show how interactions between chromatin landscapes and TF expression patterns may work together to determine gene expression programs in rat DRG neurons after nerve injury.
Keyphrases
- rna seq
- gene expression
- neuropathic pain
- single cell
- chronic pain
- transcription factor
- spinal cord
- public health
- spinal cord injury
- dna methylation
- dna binding
- genome wide
- dna damage
- pain management
- oxidative stress
- peripheral nerve
- poor prognosis
- binding protein
- diabetic rats
- electronic health record
- amino acid
- protein protein
- small molecule
- long non coding rna
- genome wide identification
- machine learning
- deep learning
- cell therapy